3H85

Molecular basis for the association of PIPKI gamma-p90 with the clathrin adaptor AP-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-20
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.91841
Spacegroup name	P 64
Unit cell lengths	125.301, 125.301, 74.546
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.010 - 2.600
R-factor	0.233
Rwork	0.231
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bw8
RMSD bond length	0.008
RMSD bond angle	1.151
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.690
High resolution limit [Å]	2.600	5.600	2.600
Rmerge	0.069	0.045	0.484
Number of reflections	19090
<I/σ(I)>	15.965
Completeness [%]	92.6	99.4	49
Redundancy	3.5	3.6	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	1.4M sodium formate, 50mM NiCl, 0.1M Na-acetate, pH6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K