3H6I
Crystal Structure of Mycobacterium Tuberculosis Proteasome Modified by inhibitor GL1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-19 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 171.615, 117.667, 196.335 |
| Unit cell angles | 90.00, 113.65, 90.00 |
Refinement procedure
| Resolution | 29.750 - 2.430 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fhg |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.750 | 2.470 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Rmerge | 0.080 | 0.333 |
| Number of reflections | 261485 | |
| <I/σ(I)> | 14 | 3.88 |
| Completeness [%] | 97.8 | 99.3 |
| Redundancy | 4.3 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.8 | 277 | 12% PEG 6000, 60 mM sodium citrate, pH 5.8, 0.1M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
