3H3O
Glycerol Kinase H232R with Ethylene Glycol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2008-02-20 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 98.844, 104.275, 114.242 |
| Unit cell angles | 90.00, 114.13, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.300 |
| R-factor | 0.181 |
| Rwork | 0.181 |
| R-free | 0.20600 |
| Structure solution method | PHASER |
| Starting model (for MR) | 1xup |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.327 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.500 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 92379 | |
| <I/σ(I)> | 11.1 | 2.1 |
| Completeness [%] | 98.5 | 97 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | KH2PO4, PEG 8000, VAPOR DIFFUSION, temperature 298K |
