3H2V
Human raver1 RRM1 domain in complex with human vinculin tail domain Vt
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-19 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.818, 70.921, 99.342 |
| Unit cell angles | 89.77, 90.04, 104.04 |
Refinement procedure
| Resolution | 20.000 - 2.900 |
| R-factor | 0.2131 |
| Rwork | 0.210 |
| R-free | 0.27690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rke |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.203 |
| Refinement software | BUSTER-TNT (2.3.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.900 |
| Number of reflections | 23190 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 100 mM Tris (pH 7.5), 200 mM sodium nitrate, and 16% PEG-3,350, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
