3H1V
Human glucokinase in complex with a synthetic activator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL32B2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-05-25 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 79.793, 79.793, 326.124 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.460 - 2.110 |
| R-factor | 0.235 |
| Rwork | 0.232 |
| R-free | 0.28400 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.013 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.190 |
| High resolution limit [Å] | 2.110 | 4.540 | 2.110 |
| Rmerge | 0.048 | 0.029 | 0.292 |
| Number of reflections | 34817 | ||
| <I/σ(I)> | 31.93 | ||
| Completeness [%] | 94.5 | 93.9 | 83 |
| Redundancy | 5.6 | 6.2 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 298 | 30% PEG 1500, HEPES, pH 6.6, vapor diffusion, hanging drop, temperature 298K |
