3H0F
Crystal structure of the human Fyn SH3 R96W mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-01-27 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 52.103, 52.103, 53.259 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.300 - 2.610 |
| R-factor | 0.20912 |
| Rwork | 0.202 |
| R-free | 0.28226 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1shf |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.703 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.300 | 2.720 |
| High resolution limit [Å] | 2.610 | 2.610 |
| Rmerge | 0.150 | 0.630 |
| Number of reflections | 2270 | |
| <I/σ(I)> | 13.4 | 1.7 |
| Completeness [%] | 91.9 | 58.2 |
| Redundancy | 5.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.1 | 277 | 2.0M ammonium sulphate, 2% PEG 400, 0.1M Tris, pH 9.1, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
