3GW6
Intramolecular Chaperone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 62.650, 79.950, 109.580 |
| Unit cell angles | 81.71, 76.53, 87.11 |
Refinement procedure
| Resolution | 27.633 - 2.600 |
| R-factor | 0.2112 |
| Rwork | 0.209 |
| R-free | 0.25450 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.727 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.042 | 0.411 |
| Number of reflections | 184838 | |
| <I/σ(I)> | 2.2 | |
| Completeness [%] | 95.2 | 95.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 15 % PEG 8000, 0.1 M BisTris pH 6.0, 0.15 M CaBr2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
