3GVU
The crystal structure of human ABL2 in complex with GLEEVEC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-30 |
| Detector | MAR225 |
| Wavelength(s) | 0.88000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 88.460, 99.440, 87.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.900 - 2.050 |
| R-factor | 0.19135 |
| Rwork | 0.189 |
| R-free | 0.22644 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1FPU.pdb |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.474 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0089) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.900 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.086 | 0.445 |
| Number of reflections | 24490 | |
| <I/σ(I)> | 12.8 | 3.2 |
| Completeness [%] | 99.3 | 95.5 |
| Redundancy | 4.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 20% PEG3350, 0.1M citrate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
