3GVR

Single-chain UROD Y164G (GY) mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-08-18
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	103.447, 103.447, 70.716
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	14.730 - 2.200
R-factor	0.197
Rwork	0.194
R-free	0.24600
RMSD bond length	0.012
RMSD bond angle	1.243
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.280
High resolution limit [Å]	2.200	4.740	2.200
Rmerge	0.110	0.069	0.395
Number of reflections	22288
<I/σ(I)>	15.353
Completeness [%]	99.3	96.1	98.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop			hanging drop