3GVP
Human SAHH-like domain of human adenosylhomocysteinase 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-01 |
| Detector | ADSC QUANTUM Q315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 164.188, 164.188, 184.614 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.910 - 2.250 |
| R-factor | 0.18231 |
| Rwork | 0.182 |
| R-free | 0.22581 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d64 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.513 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.910 | 2.370 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.104 | 0.507 |
| Number of reflections | 119253 | |
| <I/σ(I)> | 33.2 | 9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 29.6 | 29.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1M HEPES, 12.5% PEG3350, 0.2M sodium malonate, pH7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
