3GVO
Structure and RNA binding of the mouse Pumilio-2 Puf Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-30 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.05 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 142.393, 142.393, 112.233 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.720 - 1.600 |
| R-factor | 0.168 |
| Rwork | 0.167 |
| R-free | 0.19800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ib2 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.347 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.045 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.067 | 0.393 |
| Total number of observations | 15337 | |
| Number of reflections | 56410 | |
| <I/σ(I)> | 15 | 1.7 |
| Completeness [%] | 98.3 | 92.3 |
| Redundancy | 5.4 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 277 | 0.2M ammonium citrate, 20%(w/v) PEG 3350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
