3GVD
Crystal Structure of Serine Acetyltransferase CysE from Yersinia pestis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97937 |
| Spacegroup name | P 1 |
| Unit cell lengths | 68.472, 81.228, 139.406 |
| Unit cell angles | 97.07, 95.32, 94.72 |
Refinement procedure
| Resolution | 45.986 - 2.400 |
| R-factor | 0.2022 |
| Rwork | 0.199 |
| R-free | 0.26490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t3d |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.160 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.170 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.095 | 0.383 |
| Number of reflections | 112894 | |
| <I/σ(I)> | 6.1 | 2.2 |
| Completeness [%] | 97.5 | 96.7 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2 M Potassium acetate, 20% w/v Polyethylene glycol 3,350, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
