3GV5

Human DNA polymerase iota in complex with T template DNA and incoming ddADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Collection date	2008-03-07
Wavelength(s)	0.98322
Spacegroup name	C 1 2 1
Unit cell lengths	140.160, 71.750, 127.420
Unit cell angles	90.00, 112.53, 90.00

Refinement procedure

Resolution	51.570 - 2.000
R-factor	0.206
Rwork	0.205
R-free	0.25300
RMSD bond length	0.016
RMSD bond angle	1.958
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	51.570	50.000	1.980
High resolution limit [Å]	1.950	5.290	1.950
Rmerge	0.072	0.024	0.617
Number of reflections	84496
<I/σ(I)>	24.607
Completeness [%]	99.0	98.2	96.3
Redundancy	3.6	3.7	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	7.5	298	15% PEG 3350, 0.15 M CaCl2, 5% glycerol, 0.005M DTT, pH 7.5, hanging drop, temperature 298K