3GUI
T4 lysozyme M102E/L99A mutant with buried charge in apolar cavity--Apo structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-07 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 48.769, 48.769, 128.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.840 - 1.450 |
| R-factor | 0.18558 |
| Rwork | 0.185 |
| R-free | 0.20648 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dmv |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.351 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.600 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.036 | 0.280 |
| Number of reflections | 28430 | |
| <I/σ(I)> | 42 | 6.3 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.7 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 30% PEG 8000, 0.20M ammonium sulfate, 0.10M Tris, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
