3GSN
Crystal structure of the public RA14 TCR in complex with the HCMV dominant NLV/HLA-A2 epitope
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.450, 77.060, 87.220 |
| Unit cell angles | 90.00, 101.95, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.800 |
| R-factor | 0.23718 |
| Rwork | 0.215 |
| R-free | 0.28987 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.962 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.117 | 0.444 |
| Number of reflections | 22991 | |
| <I/σ(I)> | 11.08 | 2.37 |
| Completeness [%] | 94.5 | 78.7 |
| Redundancy | 3.72 | 2.66 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 18-24% PEG 3350, 0.3M LiSO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
