3GOP

Crystal structure of the EGF receptor juxtamembrane and kinase domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-16
Detector	ADSC QUANTUM 4
Wavelength(s)	0.918
Spacegroup name	P 43 21 2
Unit cell lengths	62.073, 62.073, 177.158
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.330 - 2.800
R-factor	0.213
Rwork	0.208
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gs7
RMSD bond length	0.016
RMSD bond angle	1.522
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.850
High resolution limit [Å]	2.800	7.590	2.800
Rmerge	0.103	0.053	0.306
Number of reflections	9127
Completeness [%]	98.8	92.4	100
Redundancy	7.6	6.4	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	294	10% PEG3350, 0.1M KCl, 0.1M Tris pH8.5, vapor diffusion, temperature 294K