3GNX
Structure of dehydrated D-xylose isomerase from streptomyces rubiginosus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-10-10 |
| Detector | STOE |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 98.853, 87.650, 94.531 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.350 - 2.000 |
| R-factor | 0.16254 |
| Rwork | 0.160 |
| R-free | 0.20640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xig |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.615 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 94.490 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.137 |
| Number of reflections | 51870 |
| <I/σ(I)> | 8.1 |
| Completeness [%] | 98.3 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 278 | 6mM Tris-HCl, 33mg/ml crystalline suspension, 0.91M ammonium sulfate, 1mM magnesium sulfate (Hampton Research Ltd.); Soaked in xylitol solution, pH 7.0, temperature 278K |
