3GK0
Crystal structure of pyridoxal phosphate biosynthetic protein from Burkholderia pseudomallei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-03 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 1 |
| Unit cell lengths | 90.090, 91.864, 90.024 |
| Unit cell angles | 118.48, 116.81, 93.50 |
Refinement procedure
| Resolution | 46.500 - 2.280 |
| R-factor | 0.189 |
| Rwork | 0.186 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m5w |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.152 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.107 | 0.481 |
| Number of reflections | 91137 | |
| <I/σ(I)> | 12.627 | 2.1 |
| Completeness [%] | 96.5 | 85.2 |
| Redundancy | 3.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 16.4% PEG4000, 0.1 M Hepes NaOH, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
