3GJX
Crystal Structure of the Nuclear Export Complex CRM1-Snurportin1-RanGTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.90000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.173, 225.738, 163.451 |
| Unit cell angles | 90.00, 100.56, 90.00 |
Refinement procedure
| Resolution | 38.842 - 2.500 |
| R-factor | 0.2461 |
| Rwork | 0.244 |
| R-free | 0.28080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.079 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.560 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.120 | 0.506 |
| Number of reflections | 176505 | 19500 |
| <I/σ(I)> | 10.7 | 2.9 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 4.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | PEG 1000, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | PEG 1000, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
