3GDL

Crystal structure of the orotidine 5'-monophosphate decarboxylase from Saccharomyces cerevisiae complexed with 6-azauridine 5'-monophosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-09-28
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97915
Spacegroup name	C 1 2 1
Unit cell lengths	120.730, 80.356, 77.566
Unit cell angles	90.00, 125.12, 90.00

Refinement procedure

Resolution	24.900 - 1.650
R-factor	0.201
Rwork	0.200
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gdk
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	BALBES
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	1.650
Rmerge	0.067
Number of reflections	69598
Completeness [%]	95.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	30% PEG 2000, 0.1M Sodium acetate, 0.2M Ammonium sulfate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K