3GD1
Structure of an Arrestin/Clathrin complex reveals a novel clathrin binding domain that modulates receptor trafficking
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-08-29 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9833, 0.9795, 0.9791 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 228.960, 61.198, 159.840 |
Unit cell angles | 90.00, 119.32, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.500 |
R-factor | 0.214 |
Rwork | 0.214 |
R-free | 0.25400 |
Structure solution method | MAD |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.650 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.069 | 0.236 |
Number of reflections | 25549 | |
<I/σ(I)> | 20.2 | 6.3 |
Completeness [%] | 99.1 | 91.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 9 | 293 | 100mM Bicine, 7% O-(2-aminopropyl)-O'-(2-methoxyethyl)polypropylene glycol 500, 6% polyethylene glycol 8000, 4% acetone, 1% ethylene glycol and 10 mM strontium chloride, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |