3GCP
Human P38 MAP Kinase in Complex with SB203580
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2008-05-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.01630 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.890, 69.870, 74.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.250 |
| R-factor | 0.201 |
| Rwork | 0.197 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ewa |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.662 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.350 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.046 | 0.232 |
| Number of reflections | 17526 | 2035 |
| <I/σ(I)> | 25.39 | 5.3 |
| Completeness [%] | 99.4 | 96.6 |
| Redundancy | 4.49 | 2.49 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 293 | 100 mM MES, 20-30% PEG4000, 50 mM n-BOG, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
