3GCM
Crystal Structure of E. coli polynucleotide phosphorylase bound to RNA and RNase E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2006-10-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 176.335, 176.335, 189.628 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.16911 |
| Rwork | 0.166 |
| R-free | 0.22065 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e3h |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.939 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.619 | |
| Number of reflections | 97645 | |
| <I/σ(I)> | 2.2 | |
| Completeness [%] | 99.3 | 99.5 |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293.15 | 0.2 M ammonium hydrogen citrate, 17 % w/v PEG 3350, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
