3G6W
Asymetric GTP bound structure of UPRTase from Sulfolobus solfataricus containing PRPP-mg2+ in half of the active sites and R5P and PPi in the other half
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-11-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.038, 98.758, 73.528 |
| Unit cell angles | 90.00, 92.80, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.900 |
| Rwork | 0.216 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xtt |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.970 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.098 | 0.397 |
| Number of reflections | 19229 | |
| <I/σ(I)> | 14 | 3.4 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 10% PEG8000, 0.1M sodium cacodylate, pH6.5, 200mM magnesium chloride, 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
