3G6O

Crystal structure of P. aeruginosa bacteriophytochrome PaBphP photosensory core domain mutant Q188L

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2008-07-21
Detector	MAR CCD 165 MM 315
Spacegroup name	P 65
Unit cell lengths	109.786, 109.786, 189.113
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.470 - 2.850
R-factor	0.232
Rwork	0.229
R-free	0.29800
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.256
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.000
High resolution limit [Å]	2.850	2.850
Rmerge	0.045
Number of reflections	28350
Completeness [%]	99.5	99.8
Redundancy	7.3	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.6	293	0.5% PEG4000(w/v), 0.01M sodium acetate, 10mg/ml protein in dark, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K