3G6G
Equally potent inhibition of c-Src and Abl by compounds that recognize inactive kinase conformations
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9774 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.885, 63.855, 76.622 |
| Unit cell angles | 102.50, 89.40, 90.29 |
Refinement procedure
| Resolution | 43.480 - 2.310 |
| R-factor | 0.2314 |
| Rwork | 0.229 |
| R-free | 0.27670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oiq |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.536 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0035) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 33115 | |
| Completeness [%] | 95.6 | 97.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 293 | 100 mM MES pH 6.3, 5 % PEG 3350, 10 % glycerol, 30 mM sodium acetate, 3 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
