3G65

Crystal Structure of the Human Rad9-Rad1-Hus1 DNA Damage Checkpoint Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Detector technology	CCD
Collection date	2007-04-07
Detector	ADSC QUANTUM 210
Wavelength(s)	0.934
Spacegroup name	P 1 21 1
Unit cell lengths	73.246, 70.673, 83.151
Unit cell angles	90.00, 99.51, 90.00

Refinement procedure

Resolution	82.000 - 2.900
R-factor	0.2236
Rwork	0.221
R-free	0.27240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1axc
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.000	3.060
High resolution limit [Å]	2.900	2.900
Rmerge	0.110	0.320
Number of reflections	18454
<I/σ(I)>	3.1	2.2
Completeness [%]	97.6	94
Redundancy	4.5	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.1M Tris-HCl, 17% PEG 2000-Mme, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.1M Tris-HCl, 17% PEG 2000-Mme, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K