3G5D
Kinase domain of cSrc in complex with Dasatinib
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2008-10-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.99990 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.190, 63.690, 74.580 |
| Unit cell angles | 78.71, 89.96, 90.28 |
Refinement procedure
| Resolution | 35.050 - 2.200 |
| R-factor | 0.207 |
| Rwork | 0.206 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oiq |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.549 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.054 | 0.145 |
| Number of reflections | 37432 | 4637 |
| <I/σ(I)> | 18.75 | 9.7 |
| Completeness [%] | 96.9 | 96.6 |
| Redundancy | 3.7 | 3.69 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 10% PEG 20000, 15% glycerol, 85 mM MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
