3G5C
Structural and biochemical studies on the ectodomain of human ADAM22
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | H 3 |
| Unit cell lengths | 122.090, 122.090, 208.690 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.360 |
| Rwork | 0.245 |
| R-free | 0.27270 |
| Structure solution method | combined with SIRAS |
| Starting model (for MR) | 1R54 AND 2ERO |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.490 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.069 | 0.389 |
| Number of reflections | 49760 | |
| <I/σ(I)> | 11.2 | 2.44 |
| Completeness [%] | 96.6 | 91.5 |
| Redundancy | 3.2 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
