3G43

Crystal structure of the calmodulin-bound Cav1.2 C-terminal regulatory domain dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.95372
Spacegroup name	P 21 21 21
Unit cell lengths	39.790, 113.980, 182.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.930 - 2.100
R-factor	0.22
Rwork	0.220
R-free	0.26900
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.000
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.041	0.278
Number of reflections	49200
<I/σ(I)>	39.6	5.3
Completeness [%]	99.8	99.9
Redundancy	5.9	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	20% PEG 6000, 1.0 M LiCl, 3% w/v MPD, 100 mM MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	6	298	20% PEG 6000, 1.0 M LiCl, 3% w/v MPD, 100 mM MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K