3FZZ
Structure of GrC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-05-24 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 61 |
| Unit cell lengths | 71.488, 71.488, 206.696 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.670 - 2.500 |
| R-factor | 0.217 |
| Rwork | 0.215 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a1u |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.456 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.5) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 61.911 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.064 |
| Number of reflections | 20641 |
| <I/σ(I)> | 9.484 |
| Completeness [%] | 99.8 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 298 | 0.2M ammonium sulfate, 0.25g/ml PEG 3350, 0.1M sodium cacodylate. Crystals were grown by mixing 2 micro-l of reservoir solution with 2 micro-l of protein solution (20mg/ml in 50mM HEPES pH 6.8, 200mM NaCl), VAPOR DIFFUSION, HANGING DROP, temperature 298K |
