3FY7
Crystal structure of homo sapiens CLIC3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-05 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.289, 48.673, 60.154 |
| Unit cell angles | 69.11, 80.97, 74.80 |
Refinement procedure
| Resolution | 28.030 - 1.950 |
| R-factor | 0.234 |
| Rwork | 0.231 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ahe |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.051 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.300 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.086 | 0.464 |
| Number of reflections | 33742 | |
| <I/σ(I)> | 8.6 | 1.9 |
| Completeness [%] | 94.0 | 86.6 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | SOLUTION (15 MG/ML PROTEIN IN 20 MM HEPES*NAOH, PH 7.5, 200MM NACL) PLUS 3UL OF RESERVOIR SOLUTION (1.05M NH4SO4,0.225M LISO4, 0.1M TRIS-HCL PH 8.5), DROP EQUILIBRATED AGAINST 1 ML RESERVOIR SOLUTION , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
