3FY2

Human EphA3 Kinase and Juxtamembrane Region Bound to Substrate KQWDNYEFIW

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-11-02
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54178
Spacegroup name	P 1 21 1
Unit cell lengths	53.824, 38.261, 76.365
Unit cell angles	90.00, 102.05, 90.00

Refinement procedure

Resolution	26.730 - 1.800
R-factor	0.181
Rwork	0.179
R-free	0.21200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fxx
RMSD bond length	0.011
RMSD bond angle	1.259
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.25)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.040	34.040	1.880
High resolution limit [Å]	1.780	5.630	1.780
Rmerge	0.049	0.035	0.224
Total number of observations		3476	14744
Number of reflections	29456
<I/σ(I)>	7.855	14.1	3.3
Completeness [%]	100.0	99.4	100
Redundancy	3.6	3.5	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	20 mg/mL Protein, 25% PEG 3350, 0.04M Ammonium sulfate, 0.1M Tris, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K