3FWO
The large ribosomal subunit from Deinococcus radiodurans complexed with Methymycin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.873 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 172.499, 415.691, 701.729 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.710 |
R-factor | 0.278 |
Rwork | 0.278 |
R-free | 0.33700 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 3.700 |
Number of reflections | 251032 |
<I/σ(I)> | 6.4 |
Completeness [%] | 94.7 |
Redundancy | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 291 | HEPES, NH4CL, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 291 | HEPES, NH4CL, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |