3FVG
Crystal structure of the human glutamate receptor, GluR5, ligand-binding core in complex with MSVIII-19 in space group P1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-11-25 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.627, 50.860, 62.931 |
| Unit cell angles | 80.41, 84.16, 62.06 |
Refinement procedure
| Resolution | 27.240 - 1.500 |
| R-factor | 0.16925 |
| Rwork | 0.168 |
| R-free | 0.19184 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fvn |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.266 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 79952 | |
| <I/σ(I)> | 32 | 4.54 |
| Completeness [%] | 96.9 | 93.8 |
| Redundancy | 3.9 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 303 | 35% PEG3350, 0.3M LiSO4, 5mM MSVIII-19, pH5.5, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
