Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1992-01 |
| Detector | FUJI |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 126.500, 191.700, 149.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.200 |
| R-factor | 0.232 |
| Rwork | 0.232 |
| R-free | 0.27600 |
| Structure solution method | MIR |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.600 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | CCP4 |
| Refinement software | CNS (DEVELOPMENTAL) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.072 |
| Total number of observations | 436000 * |
| Number of reflections | 83821 |
| Completeness [%] | 86.0 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 22 * | PH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | PIPES | 20 (mM) | |
| 3 | 1 | drop | 0.02 (%) | ||
| 4 | 1 | reservoir | PEG3000 | 15 (%) | |
| 5 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 100 (mM) |
