3FQ9
Design of an insulin analog with enhanced receptor-binding selectivity. Rationale, structure, and therapeutic implications
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-05-26 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.948 |
| Spacegroup name | H 3 |
| Unit cell lengths | 81.841, 81.841, 33.518 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.920 - 1.350 |
| Rwork | 0.198 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ins |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.920 | 1.430 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.039 | 0.123 |
| Number of reflections | 18052 | |
| <I/σ(I)> | 20.2 | 9.9 |
| Completeness [%] | 98.1 | 93 |
| Redundancy | 4.1 | 2.88 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.8 | 298 | 0.02M Tris, o.o5M soduim citrate, 5%acetone,0.03% phenol,0.01% zinc acetone, pH 7.8, VAPOR DIFFUSION, temperature 298K |
