3FMP
Crystal structure of the nucleoporin Nup214 in complex with the DEAD-box helicase Ddx19
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.369, 112.915, 142.573 |
| Unit cell angles | 90.00, 89.86, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.190 |
| R-factor | 0.25114 |
| Rwork | 0.249 |
| R-free | 0.28317 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fmo |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.471 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 29717 | |
| <I/σ(I)> | 14.3 | 3.6 |
| Completeness [%] | 91.3 | 70.9 |
| Redundancy | 6.8 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 298 | PEG 3000, CHES buffer, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
