3FME

Crystal Structure of Human Mitogen-Activated Protein Kinase Kinase 6 (MEK6) Activated Mutant (S207D, T211D)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-07
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0000
Spacegroup name	P 31 2 1
Unit cell lengths	132.756, 132.756, 45.719
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.000 - 2.260
R-factor	0.213
Rwork	0.212
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 2DYL 3eqb 1s9j
RMSD bond length	0.013
RMSD bond angle	1.308
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.2)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.320	2.380
High resolution limit [Å]	2.260	2.260
Rmerge	0.082	0.812
Number of reflections	21933
<I/σ(I)>	14.2	2.8
Completeness [%]	100.0	100
Redundancy	9.2	8.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.9	277	0.05M Mg(COO)2 10w/v PEG_3350, pH 5.9, VAPOR DIFFUSION, SITTING DROP, temperature 277K