3FLO
Crystal structure of the carboxyl-terminal domain of yeast DNA polymerase alpha in complex with its B subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-16 |
| Detector | ADSC Q315R |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.411, 142.633, 175.255 |
| Unit cell angles | 90.00, 102.33, 90.00 |
Refinement procedure
| Resolution | 31.900 - 2.500 |
| R-factor | 0.192 |
| Rwork | 0.191 |
| R-free | 0.21800 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.545 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.900 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.102 | 0.652 |
| Number of reflections | 141597 | |
| <I/σ(I)> | 5.2 | 1.2 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 7.3 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 0.1M MES, 1.26M Ammonium Sulfate, 3mM TCEP, pH6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
