3FLC
Crystal structure of the His-tagged H232R mutant of glycerol kinase from Enterococcus casseliflavus with glycerol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-17 |
| Wavelength(s) | 0.931 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 92.559, 192.234, 57.815 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.850 |
| R-factor | 0.2018 |
| Rwork | 0.202 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r59 molecule O |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.310 |
| Data reduction software | DENZO (1.96.2) |
| Data scaling software | SCALEPACK (1.96.2) |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 87047 | |
| <I/σ(I)> | 16.2 | 2.15 |
| Completeness [%] | 97.8 | 97.8 |
| Redundancy | 18.25 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 2.0M ammonium sulfate, 5%(v/v) isopropanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
