3FKU
Crystal structure of influenza hemagglutinin (H5) in complex with a broadly neutralizing antibody F10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-21 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 205.300, 118.500, 338.900 |
| Unit cell angles | 90.00, 99.60, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.200 |
| R-factor | 0.23461 |
| Rwork | 0.232 |
| R-free | 0.28623 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.312 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.280 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.128 | 0.900 |
| Number of reflections | 112570 | |
| <I/σ(I)> | 1.95 | |
| Completeness [%] | 85.0 | 68.4 |
| Redundancy | 4.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 12.5% PEG 1K, 25% ethylene glycol, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
