3FJT
Crystal structure of a human Fc fragment engineered for extended serum half-life
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-05-06 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.660, 79.605, 145.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.500 |
| R-factor | 0.23 |
| Rwork | 0.227 |
| R-free | 0.29100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.412 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.739 | 2.580 |
| High resolution limit [Å] | 2.492 | 2.492 |
| Rmerge | 0.100 | 0.440 |
| Number of reflections | 19236 | |
| <I/σ(I)> | 7.4 | 2.7 |
| Completeness [%] | 92.4 | 93.2 |
| Redundancy | 3.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 15% PEG 6000, 5% MPD, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
