3FIG
Crystal Structure of Leucine-bound LeuA from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.207, 154.258, 68.669 |
| Unit cell angles | 90.00, 97.99, 90.00 |
Refinement procedure
| Resolution | 27.660 - 2.300 |
| R-factor | 0.17 |
| Rwork | 0.166 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sr9 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.029 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 49393 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | Sodium Citrate, MPEG 2000, pH 6.0, Vapor diffusion, sitting drop, temperature 291K |
