3FHG
Crystal structure of Sulfolobus solfataricus 8-oxoguanine DNA glycosylase (SsOgg)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-19 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 6 |
| Unit cell lengths | 110.960, 110.960, 34.410 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.700 - 1.900 |
| R-factor | 0.226 |
| Rwork | 0.207 |
| R-free | 0.24800 |
| Starting model (for MR) | 3fhf |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.085 | 0.536 |
| Number of reflections | 37358 | 5344 |
| <I/σ(I)> | 17.6 | 2.3 |
| Completeness [%] | 95.9 | 100 |
| Redundancy | 5.7 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 285 | 50 mM sodium cacodylate, 2M (NH4)2SO4, 10mM MgSO4, (from four years old crystal tray), pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
