3FFV
Crystal Structure Analysis of Syd
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-09 |
| Detector | MARMOSAIC 325 MM CCD MARMOSAIC 325 MM CCD |
| Spacegroup name | H 3 |
| Unit cell lengths | 167.600, 167.600, 41.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.900 - 2.000 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| R-free | 0.23000 |
| Structure solution method | SAD |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.068 | 0.151 |
| Number of reflections | 29401 | |
| <I/σ(I)> | 8.8 | |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 5.6 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 298 | 0.8-1.0 M sodium citrate, 0.2 M sodium chloride, 0.1 M Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K | |
| 1 | 7 | 298 | 0.8-1.0 M sodium citrate, 0.2 M sodium chloride, 0.1 M Tris, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
