3FF7
Structure of NK cell receptor KLRG1 bound to E-cadherin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.502 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.120, 83.411, 56.508 |
Unit cell angles | 90.00, 106.10, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.800 |
Rwork | 0.214 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ff7 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.559 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.710 |
High resolution limit [Å] | 1.650 | 3.550 | 1.650 |
Rmerge | 0.064 | 0.058 | 0.563 |
Number of reflections | 57098 | ||
<I/σ(I)> | 21.542 | ||
Completeness [%] | 98.6 | 98.8 | 88.3 |
Redundancy | 7.3 | 9.6 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.6 | 298 | PEG4000, pH 4.6, vapor diffusion, temperature 298K |