3FEM
Structure of the synthase subunit Pdx1.1 (Snz1) of PLP synthase from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.931 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 154.073, 154.218, 154.868 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.020 |
| R-factor | 0.16267 |
| Rwork | 0.162 |
| R-free | 0.18097 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nv1 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.284 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.050 |
| High resolution limit [Å] | 3.020 | 3.020 |
| Rmerge | 0.075 | 0.416 |
| Number of reflections | 72026 | |
| <I/σ(I)> | 23.4 | 3.4 |
| Completeness [%] | 99.8 | 96.2 |
| Redundancy | 7.9 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 4M formate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
