3FDY
Pyranose 2-oxidase thermostable triple mutant, T169G/E542K/V546C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 101.580, 101.580, 126.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.800 - 1.550 |
| R-factor | 0.18703 |
| Rwork | 0.186 |
| R-free | 0.21883 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2igo |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.981 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.300 | 1.600 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 1581896 | |
| <I/σ(I)> | 12.8 | 2.8 |
| Completeness [%] | 99.8 | 97.5 |
| Redundancy | 16.4 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 298 | 10% (w/v) monomethylether PEG 2000, 0.1M Mes pH 5.2, 50mM MgCl2, 25% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
