3FC2
PLK1 in complex with BI6727
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 66.616, 66.616, 153.969 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.180 - 2.450 |
| R-factor | 0.184 |
| Rwork | 0.183 |
| R-free | 0.22600 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.222 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.580 | |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.094 | 0.568 |
| Number of reflections | 14942 | 2123 |
| <I/σ(I)> | 5.4 | |
| Completeness [%] | 98.3 | 98.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | VAPOR DIFFUSION, HANGING DROP, temperature 277K |
